Journal: Cell Death Discovery

Article Title: SLC26A9 promotes colorectal tumorigenesis by modulating Wnt/β-catenin signaling

doi: 10.1038/s41420-024-01888-6

Figure Lengend Snippet: SLC26A9 mRNA and protein were detected in CRC cell lines by A RT‒qPCR and B western blotting analysis. Each bar represents the mean ± SD. C Cell proliferation detected by CCK-8 assay, D cell growth determined by the growth curve assay, E Ki-67 staining, scale bars = 100 μm, F colony formation experiment, scale bars = 25 μm, G Cell migration was detected by wound healing assay, scale bars = 200 μm, H cell apoptosis determined by flow cytometry. * p < 0.05, ** p < 0.01 and **** p < 0.0001, ns not significantly different, compared with relevant controls, n = 3–6 in each series.

Article Snippet: Normal colonic mucosal NCM460 cells and CRC cell lines (SW48 and COLO 201) were purchased from the Cell Bank of Type Culture Collection of BeNa Culture Collection (from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, CCK-8 Assay, Staining, Migration, Wound Healing Assay, Flow Cytometry

Journal: Oncotarget

Article Title: Regorafenib in combination with silybin as a novel potential strategy for the treatment of metastatic colorectal cancer

doi: 10.18632/oncotarget.20054

Figure Lengend Snippet: The SW48, SW48-CR, HCT15 and SW480 cancer cells were treated with silybin (90 μM) or regorafenib (2 μM) and their combination for 72 hours. After 14 days the colonies were stained with 0.1% crystal violet and counted as described in Materials and Methods. ( A ) Images of colon cancer cells acquired by phase-contrast microscope. ( B ) Histogram of colony number counted by image j plugin. Error bars indicate the standard deviation. * p < 0.05 compared to single treatment. The effects of treatments were also evaluated in terms of oxidative stress generation as described in Materials and Methods. ( C ) Histogram of DHE mean fluorescence intensity (% of control). Error bars indicate the standard deviation. * p < 0.05 compared to positive control. ( D ) Flow cytometry overlay of dihydroethidium (DHE) fluorescence intensity within HCT15 cells.

Article Snippet: The human SW48 colon cancer cell ( KRAS, NRAS, BRAF and PIK3CA wild-type profile) was obtained from IRCCS “Azienda Ospedaliera Universitaria San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genova” Italy.

Techniques: Staining, Microscopy, Standard Deviation, Fluorescence, Control, Positive Control, Flow Cytometry

Journal: Oncotarget

Article Title: Regorafenib in combination with silybin as a novel potential strategy for the treatment of metastatic colorectal cancer

doi: 10.18632/oncotarget.20054

Figure Lengend Snippet: ( A ) Apoptosis was evaluated with Annexin-V-FITC staining and 7-Amino-Actinomycin D (7-AAD) detection assays using flow cytometry in SW48, SW48-CR, HCT15 and SW480 cancer cells after 24 hours of incubation with silybin (90 μM) or regorafenib (2 μM) and their combination. Histogram of data expressed as percentage of apoptotic cells.* p < 0.05 compared to single treatment. ( B ) Colon cancer cells were treated with silybin (90 μM) or regorafenib (2 μM) and their combination for 24 hours. Expression of PARP, caspase 3 and 9 were evaluated by immunoblotting as described in Materials and Methods. α-Tubulin was used as the loading control.

Article Snippet: The human SW48 colon cancer cell ( KRAS, NRAS, BRAF and PIK3CA wild-type profile) was obtained from IRCCS “Azienda Ospedaliera Universitaria San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genova” Italy.

Techniques: Staining, Flow Cytometry, Incubation, Expressing, Western Blot, Control

Journal: Oncotarget

Article Title: Regorafenib in combination with silybin as a novel potential strategy for the treatment of metastatic colorectal cancer

doi: 10.18632/oncotarget.20054

Figure Lengend Snippet: SW48, SW48-CR, HCT15 and SW480 cancer cells were treated with silybin or regorafenib and their combination at the indicated doses for 24 hours. Total cell protein extracts were subjected to immunoblotting with the indicated antibodies as described in Materials and Methods. α-Tubulin was used as the loading control.

Article Snippet: The human SW48 colon cancer cell ( KRAS, NRAS, BRAF and PIK3CA wild-type profile) was obtained from IRCCS “Azienda Ospedaliera Universitaria San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genova” Italy.

Techniques: Western Blot, Control

Journal: Cancer Science

Article Title: KHDRBS3 promotes multi‐drug resistance and anchorage‐independent growth in colorectal cancer

doi: 10.1111/cas.14805

Figure Lengend Snippet: Targeted knockout of KHDRBS3 in CRC cell lines. A, Western blotting analysis confirmed the knockout of KHDRBS3 . B, Dose‐response curves of HCT‐116 and LoVo transfected with empty vector (EV) or KHDRBS3 ‐specific knockout vector (KO) that were treated with 5‐FU, oxaliplatin (L‐OHP) and trifluridine (FTD). Error bars represent SD. ** P < .01 from Student t test. NS: P > .05 from Student t test. C, mRNA expression levels of CD44v, MRP1, and CD44s on HCT‐116 and LoVo transfected with EV or KO. Error bars represent SD. ** P < .01 from Student t test. NS: P > .05 from Student t test

Article Snippet: Ten cell lines derived from human CRC (DLD‐1, Colo‐201, RKO, WiDr, LoVo, HCT‐116, HT‐29, SW‐480, SW‐48 and SW‐837) were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Knock-Out, Western Blot, Transfection, Plasmid Preparation, Expressing

Journal: Cancer Science

Article Title: KHDRBS3 promotes multi‐drug resistance and anchorage‐independent growth in colorectal cancer

doi: 10.1111/cas.14805

Figure Lengend Snippet: Overexpression of KHDRBS3 in CRC cell lines. A, Western blotting analysis confirmed the overexpression of KHDRBS3. B, Dose‐response curves of RKO and WiDr transfected with empty vector (EV) or KHDRBS3‐specific overexpression vector (OE) that were treated with 5‐FU, oxaliplatin (L‐OHP) and trifluridine (FTD). Error bars represent SD. ** P < .01 from Student t test. NS: P > .05 from Student t test. C, mRNA expression levels of CD44v, MRP1, and CD44s on RKO and WiDr transfected with EV or OE. Error bars represent SD. ** P < .01 from Student t test. NS: P > .05 from Student t test

Article Snippet: Ten cell lines derived from human CRC (DLD‐1, Colo‐201, RKO, WiDr, LoVo, HCT‐116, HT‐29, SW‐480, SW‐48 and SW‐837) were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Over Expression, Western Blot, Transfection, Plasmid Preparation, Expressing

Journal: Cancer Science

Article Title: KHDRBS3 promotes multi‐drug resistance and anchorage‐independent growth in colorectal cancer

doi: 10.1111/cas.14805

Figure Lengend Snippet: Immunohistochemistry and Kaplan‐Meier curves for KHDRBS3 in human CRC tissues. A, Representative images of immunohistochemical staining of KHDRBS3 in tissue sections from human colorectal carcinoma (original magnification ×40 [left] and ×400 [right]). Red box: non‐tumorous area, blue box: tumorous area. B, Representative images of upper: H&E staining and lower: immunohistochemical staining of KHDRBS3 in well (Case 1: tub1) and moderately differentiated (Case 2: tub2) CRC (original magnification ×100). C, Survival analysis of KHDRBS3 in 148 CRC cases (Cohort 1). The P ‐values (log‐rank test) are shown for the survival analysis. P < .05 indicates statistical significance

Article Snippet: Ten cell lines derived from human CRC (DLD‐1, Colo‐201, RKO, WiDr, LoVo, HCT‐116, HT‐29, SW‐480, SW‐48 and SW‐837) were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Immunohistochemistry, Immunohistochemical staining, Staining